The New Prep-Runner 96™
INSTRUMENT - Prep Runner 96
Weight: uncrated: 26 lb. (11.8 kg)
Height: 9 in. (23 cm.)
Width: 14 in. 31.5 cm
Depth: 19.5 in. (49 cm.)
Work area recommended: 16x24 in. (40x60 cm.)
Operation Requirements:
110 volts AC 60 Hz (USA and Canada)
100 volts (Japan)
200-240 volts AC 50/60 Hz (Europe)
Operating Temperature Range: 18 - 37 °C
DISPOSABLE CASSETTE
| Well capacity: | 12 wells (2ml) | 24 wells (1ml) |
| Spacing of loading & sample wells: |
0.354 in. | .177 in. |
| Finished Sample Volume: |
20 - 25 µl | 20 - 25 µl |
| Separation material: | treated agarose | treated agarose |
| Samples per program: | 1-48 | 1-96 |
MICROPROCESSOR-Controlled Electrophoresis System
Voltage Range: 0 - 180 volt (direct current)
Current Range: 0 - 1.0 amp
Power Range: 0 - 180 watts
Programmable Output - Voltage and time segments of run can be programmed by operator
PLASMID SIZE RANGE
1 - 15 kb on programs 1, 2, or 3
1 - 20 kb with user input program
Typical Yield Data from various E.coli strains (Yield Increase 1.5 to 2 fold with the 2 ml cassettes) |
||||
| E.coli cell line | Yield with Bluescript* | E.coli cell line | Yield with Bluescript* | |
| 1. DH10B | 3-6 µg | 10. INV1α | 3-6 µg | |
| 2. DH5αF' | 3-6 µg | 11. SURE | 2-3 µg | |
| 3. DH5α | 3-6 µg | 12. DH11S | 3-6 µg | |
| 4. DH5 | 3-6 µg | 13. TOP 10F' | 3-5 µg | |
| 5. JM109 | 2-6 µg | 14. TOP 10 | 3-6 µg | |
| 6. HB101 | 3-5 µg | 15. XL-1 Blue (1994) | 3-6 µg | |
| 7. LE392 | 3-6 µg | 16. SOLR | 0.1 µg | |
| 8. MC1061 | 3-6 µg | 17. RR1 | 1 µg | |
| 9. INV1αF' | 3-6 µg | 18. BB4 | 2-6 µg | |
| *All DNA >95% super-coiled | ||||
Disposables
Sample Cassette, Running Buffer, Lysis Buffer (A and B) or Applicator Combs, Powdered Bacterial Growth Media
Post Manipulations
Manual Sequencing, Automated Fluorescent Sequencing, Restriction Digest, DNA Labeling, Transformation, RNA Transcription, Ligation, DNA Amplification
